Target Name: Human Her2; Human ERBB2, CD340
Source: Recombinant Human Her2 protein with C-terminus His tag & Avi tag is expressed in CHO cell. (Accession number P04626-1) (Thr23-Thr652). This protein was site-specifically labeled with Biotin by BirA ligase.
Molecular Weight: The 663 amino acid protein has the predicted molecular weight of 73.0 kDa. The protein migrates at approximately 100 and 85 kDa on SDS-PAGE in DTT-reduced and non-reduced condition, respectively.
Purity: >90% based on SDS-PAGE.
Formulation:   1XPBS, pH7.4, , 0.22 µm filtered.
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot-specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.