Target Name: 
Human Mesothelin Protein; MSLN; MPF
Source: 
Human Mesothelin protein (Accession # AAH09272.1) (Glu296-Gly580) with C-terminus His tag & Avi tag is expressed in HEK293 cells. This protein was site-specifically labeled with Biotin by BirA ligase. The labeling efficiency is greater than 90% by gel shift assay.
Molecular Weight: 
The protein has a predicted molecular weight of 33.7 kDa and migrates at approximately 35-45 kDa on SDS-PAGE with DTT-reduced conditions.
Purity: 
>95% based on SDS-PAGE.
Formulation:   
1xPBS, pH7.4, 0.22 µm filtered.
Vial Size:
25µg, 100µg
Protein Concentration: 
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: 
Quick spin the vial after receiving. An unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.