BT-plex™ Human Anti-SARS CoV2 Spike Protein Antibody kit is a bead-based immunoassay, using a magnetic fluorescence-encoded bead optimized for use on regular flow cytometers. The assay simultaneously quantifies specific human anti-SARS CoV-2 spike protein IgG, IgM, and IgA in the same sample. The assay has been analytically validated for use on serum/plasma samples. The assay kit provides better detection sensitivities, broader dynamic ranges, and less hands-on time than traditional ELISA methods.
Kit Components: Magnetic beads immobilied with S Trimer protein, Standard cocktail containing human anti-SARS CoV2 RBD monoclonal antiboies with IgG, IgM, and IgA isotype, Detection antibodies for human IgG, IgM, and IgA isotypes, SAPE reporter, Assay buffer, Wash Buffer, V-bottom 95-well plate, and plate sealers
Size: 100 tests
Sample type: serum/plasma (1:500) and other sample types
Protocol time: 3.5 hours
Limit of detection: 5 pg/mL for IgG, IgM; 2.5 pg/mL for IgA
Dynamic range: 6 pg/mL to 25 ng/mL
Accuracy: 80-100%
Precisions: Intra-assay CV < 10%; Inter-assay CV < 15%
Anti-SARS-CoV-2 Spike Protein Human IgA/IgG/IgM Flow Test Kit (BT-plex)
Catalog Number: X202001
Kit Components:
Kit Components Quantity Volume SARS CoV-2 Spike Trimer Beads 1 Bottle 6.0 mL SARS CoV-2 Antibody Standard 1 Vial 0.5 mL Human IgG Detection Antibody 1 Bottle 2.0 mL Human IgM Detection Antibody 1 Bottle 2.0 mL Human IgA Detection Antibody 1 Bottle 2.0 mL BT-plex Streptavidin-Phycoerythrin 1 Bottle 6.0 mL Assay Buffer 2 Bottle 25 mL 20X Wash Buffer 1 Bottle 25 mL Low-binding V-bottom plate 1 Plate NA Adhesive Plate Sealer 2 Sheets NA Instruction manual 1