HRP Conjugated Anti-His antibody (Clone BR20M03)

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His tag is one of the commonly used affinity tags for recombinant protein expression and purification. BioTimes’ His tag antibody is raised against the 6xHis synthetic peptide. It can be used for ELISA as the detection antibody or WB for recombinant protein with His tag at either C-terminus, N-terminus, or internally. It might be used for other applications like IHC or immunofluorescent, but these applications have not been tested in house.

For ELISA and WB applications, it can be used at 0.1-0.5ug/mL. For ELISA and WB applications, it can be used at 0.1-0.5ug/mL. HRP conjugated antibody can be used directly for ELISA or WB without additional HRP conjugated 2nd step.