Target Name: human CTLA-4, CD152, Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4, Cytotoxic T-Lymphocyte Protein 4
Source: Human CTLA4 protein (accession number Q6GR94) (Ala37-Phe162) with C-terminus His tag is expressed in CHO cells.
Molecular Weight: The 139 amino acid protein has the predicted molecular weight of 15 kDa. The protein migrates at approximately 25 kDa and 45 kDa on SDS-PAGE in DTT-reduced and non-reduced conditions, respectively.
Purity: >90% based on SDS-PAGE
Formulation: 1xPBS buffer, 0.22 µm filtered
Endotoxin Level: Not tested
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Briefly centrifuge the vial upon receipt. An unopened vial can be kept at 4°C for up to 2 weeks or at -20°C or lower for six months. The protein may be diluted to 0.1 mg/mL using 0.22 µm filtered PBS buffer. To avoid repeated freeze/thaw cycles, aliquot the stock solution and store at ≤ -70°C. If additional dilution is required, use a carrier protein like FBS or BSA.