Target Name: Human Her2;  human ERBB2, CD340
Source: Recombinant Human Her2α protein with C-terminus His tag is expressed in CHO cells. (Accession number P04626-1) (Thr23-Thr652)
Molecular Weight: The 643 amino acid protein has a predicted molecular weight of 70.8 kDa. The protein migrates at approximately 100 and 85 kDa on SDS-PAGE in DTT-reduced and non-reduced conditions, respectively.
Purity: >90% based on SDS-PAGE.
Formulation: 1xPBS, pH7.4, 0.22 µm filtered.
Vial Size: 25µg, 100µg
Protein Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling: Quick spin the vial after receiving. An unopened vial can be stored at 2-8°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier proteins such as FBS and BSA need to be added if further dilution is needed.