Target Name: Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Source: Recombinant Human TNF alpha (accession number Q9GZX6) (Val77-Leu233) is expressed in E.coli.
Molecular Weight: The 157 amino acid protein has a predicted molecular weight of 17.5kDa. The protein migrates at approximately 16kDa on SDS-PAGE with DTT-reduced condition
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, PH7.4.
Vial Size: 10µg, 50µg
Endotoxin Level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.